Laboratory Testing of Severe Acute Respiratory Virus Coronavirus 2

A New York Institutional Experience
      Severe acute respiratory virus coronavirus 2 virus traumatized New York in March and April of 2020 and the coronavirus disease 19 (COVID-19) pandemic left an indelible mark on the majority of individuals particularly, in New York City.

      Keywords

      To read this article in full you will need to make a payment

      Subscribe:

      Subscribe to Advances in Molecular Pathology
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect

      References

      1. Severe acute respiratory syndrome coronavirus 2 isolate Wuhan-Hu-1, complete genome, in NCBI Reference Sequence: NC_045512.2. 2019. Available at: https://www.ncbi.nlm.nih.gov/nuccore/1798174254.

      2. World Health Organization Coronavirus disease (COVID-19) Pandemic.
        (Available at:) (Accessed May 26th, 2020)
        • Mayer L.M.
        • Kahlert C.
        • Rassouli F.
        • et al.
        Impact of viral multiplex real-time PCR on management of respiratory tract infection: a retrospective cohort study.
        Pneumonia (Nathan). 2017; 9: 4https://doi.org/10.1186/s41479-017-0028-z
        • Sheridan C.
        Coronavirus and the race to distribute reliable diagnostics.
        Nat Biotechnol. 2020; 38: 382-384
      3. New York State Department of Health COVID-19 Tracker.
        (Available at:) (Accessed May 26th, 2020)
        • U.S. Food and Drug Administration
        Emergency Use Authorizations.
        (Available at:) (Accessed May 26th, 2020)
      4. Prevention, C.o.D.C.a., Interim Guidelines for Collecting, Handling, and Testing Clinical Specimens from Persons for Coronavirus Disease 2019 (COVID-19). https://www.cdc.gov/coronavirus/2019-ncov/lab/guidelines-clinical-specimens.html.

        • Kim Y.-g.
        • Yun S.G.
        • Kim M.Y.
        • et al.
        Comparison between saliva and nasopharyngeal swab specimens for detection of respiratory viruses by multiplex reverse transcription-PCR.
        J Clin Microbiol. 2017; 55: 226-233
        • Tu Y.-P.
        • Jennings R.
        • Hart B.
        • et al.
        Patient-collected tongue, nasal, and mid-turbinate swabs for SARS-CoV-2 yield equivalent sensitivity to health care worker collected nasopharyngeal swabs.
        medRxiv. 2020; (04.01.20050005)
        • Kucirka L.M.
        • Lauer S.A.
        • Laeyendecker O.
        • et al.
        Variation in false-negative rate of reverse transcriptase polymerase chain reaction–based SARS-CoV-2 tests by time since exposure.
        Ann Intern Med. 2020; https://doi.org/10.7326/M20-1495
        • Wölfel R.
        • Corman V.M.
        • Guggemos W.
        • et al.
        Virological assessment of hospitalized patients with COVID-2019.
        Nature. 2020; 581: 465-469
        • Loeffelholz M.J.
        • Tang Y.-W.
        Laboratory diagnosis of emerging human coronavirus infections – the state of the art.
        Emerg Microbes Infect. 2020; 9: 747-756
        • Rogers A.A.
        • Baumann R.E.
        • Borillo G.A.
        • et al.
        Evaluation of Transport Media and Specimen Transport Conditions for the Detection of SARS-CoV-2 Using Real Time Reverse Transcription PCR.
        J Clin Microbiol. 2020; https://doi.org/10.1128/JCM.00708-20
        • Park G.-S.
        • Ku K.
        • Baek S.-H.
        • et al.
        Development of reverse transcription loop-mediated isothermal amplification assays targeting severe acute respiratory syndrome coronavirus 2.
        J Mol Diagn. 2020; 22: 729-735
        • Joung J.
        • Ladha A.
        • Saito M.
        • et al.
        Point-of-care testing for COVID-19 using SHERLOCK diagnostics.
        medRxiv. 2020; (05.04.20091231)https://doi.org/10.1101/2020.05.04.20091231
        • Smithgall M.C.
        • Scherberkova I.
        • Whittier S.
        • et al.
        Comparison of cepheid xpert xpress and abbott id now to roche cobas for the rapid detection of SARS-CoV-2.
        J Clin Virol. 2020; 128: 104428
        • Harrington A.
        • Cox B.
        • Snowdon J.
        • et al.
        Comparison of Abbott ID Now and Abbott m2000 methods for the detection of SARS-CoV-2 from nasopharyngeal and nasal swabs from symptomatic patients.
        J Clin Microbiol. 2020; https://doi.org/10.1128/JCM.00798-20
        • Mitchell S.L.
        • George K.S.
        Evaluation of the COVID19 ID NOW EUA assay.
        J Clin Virol. 2020; 128: 104429
        • Zhen W.
        • Smith E.
        • Manji R.
        • et al.
        Clinical evaluation of three sample-to-answer platforms for the detection of SARS-CoV-2.
        J Clin Microbiol. 2020; https://doi.org/10.1128/JCM.00783-20
        • Basu A.
        • Zinger T.
        • Inglima K.
        • et al.
        Performance of the rapid Nucleic Acid Amplification by Abbott ID NOW COVID-19 in nasopharyngeal swabs transported in viral media and dry nasal swabs, in a New York City academic institution.
        bioRxiv. 2020; (05.11.089896)https://doi.org/10.1128/JCM.01136-20
      5. U.S. Food and Drug Administration Beware of Fraudulent Coronavirus Tests, Vaccines and Treatments.
        (Available at:) (Accessed May 26th, 2020)
        • Zhao J.
        • Yuan Q.
        • Wang H.
        • et al.
        Antibody responses to SARS-CoV-2 in patients of novel coronavirus disease 2019.
        Clin Infect Dis. 2020; https://doi.org/10.1093/cid/ciaa344
        • To K.K.
        • Tsang O.T.
        • Leung W.S.
        • et al.
        Temporal profiles of viral load in posterior oropharyngeal saliva samples and serum antibody responses during infection by SARS-CoV-2: an observational cohort study.
        Lancet Infect Dis. 2020; 20: 565-574
        • Rokni M.
        • Ghasemi V.
        • Tavakoli Z.
        Immune responses and pathogenesis of SARS-CoV-2 during an outbreak in Iran: Comparison with SARS and MERS.
        Rev Med Virol. 2020; 30: e2107
        • Chen N.
        • Zhou M.
        • Dong X.
        • et al.
        Epidemiological and clinical characteristics of 99 cases of 2019 novel coronavirus pneumonia in Wuhan, China: a descriptive study.
        Lancet. 2020; 395: 507-513
        • Gray E.R.
        • Bain R.
        • Varsaneux O.
        • et al.
        p24 revisited: a landscape review of antigen detection for early HIV diagnosis.
        AIDS. 2018; 32: 2089-2102
        • Kaul K.L.
        Laboratories and pandemic preparedness: A framework for collaboration and oversight.
        J Mol Diagn. 2020; 22: 841-843